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The temperature and IPTG concentration for protein expression were consistent with the above conditions, and ncAA p-acetylphenylalanine (pAcF) with a final concentration of 1 mM was added

(143), NO donors such as nitroglycerin can attenuate this PD-L1 upregulation, thereby restoring T-cell cytotoxicity

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However, these described methods are not without insufficiencies that result from the complexities in IGF-1 as a peptide

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